Biodiversity Monitoring - Malaise trap transects

Responsibility: Entomological Society Krefeld (EVK)

This work package will assess insect biodiversity via standardized trapping along transects in regular intervals at 21 nature protected areas. The methodology is based on the well established and published standards by the EVK. The EVK is the only institution which is using Malaise traps similar to the one used by Townes (1972) in a particularly standardised sampling design for decades to investigate local insect diversity and other related research topics. Species identification and measurment of abundances of selected insect taxa will provide the basis for the interpretation of the results in comparison to results from metabarcoding and spatial distribution patterns as well as data from adjacent land use and chemical pollution. These tasks can only be fulfilled by the participation of a higher amount of qualified entomologists. The primary tasks of the EVK within DINA are:

Set up Malais traps at the Geesower Hügel site.

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  • Assistance in preselection and final selection of study sites with help of aerial photographs made by video drones.

  • Setup and installation of Malaise traps at 21 study sites (each locality consists of 5 traps along a transect).

  • Documentation of the study areas according to the standard established by the EVK.

  • Phytosociological records and documentation of vegetation and habitats with help of aerial photographies.

  • Weight measurments of the total wet biomass of each sample and further processing  of the sample liquids for studies on plant fragments and pollen.

  • Sorting, taxa identification and numbering of selected taxa of the most species-rich insect orders in Germany, i.e. Hymenoptera, Diptera, Coleoptera and Lepidoptera.. Since metabarcoding cannot be used for reliable estimation of taxon-specific abundances, insect tables of the orders Hymenoptera, Diptera, Coleoptera and Lepidoptera are required for morphological identification and subsequent manual counting to supplement the results obtained by metabarcoding. The abundances are determined for all transects using summer samples with peak biomass. This approach provides important insights into the possibility of obtaining information on relative species abundances and evaluating this, including data from metabarcoding.

  • Insects identified in this context will also be used to complete the GBOL reference database. Since selected families of the megadiverse insect orders Hymenoptera, Coleoptera, Lepidoptera and Diptera are manually identified by EVK specialists, we expect many species that will complement the global DNA barcode reference libraries in the work process.

  • The study will provide an enormous data volume on flight active insect species by Malaise traps of selected sites. The combination of metabarcoding and selective abundance evaluations will provide a hitherto unavailable basis for assessing the biodiversity of insect species in nature reserves in Germany.

  • Participation in the evaluation of the results and the synthesis

Contents of a sample bottle after extraction.

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  • Assistance in preselection and final selection of study sites with help of aerial photographs made by video drones.

  • Setup and installation of Malaise traps at 21 study sites (each locality consists of 5 traps along a transect).

  • Documentation of the study areas according to the standard established by the EVK.

  • Phytosociological records and documentation of vegetation and habitats with help of aerial photographies.

  • Weight measurments of the total wet biomass of each sample and further processing  of the sample liquids for studies on plant fragments and pollen.

  • Sorting, taxa identification and numbering of selected taxa of the most species-rich insect orders in Germany, i.e. Hymenoptera, Diptera, Coleoptera and Lepidoptera.. Since metabarcoding cannot be used for reliable estimation of taxon-specific abundances, insect tables of the orders Hymenoptera, Diptera, Coleoptera and Lepidoptera are required for morphological identification and subsequent manual counting to supplement the results obtained by metabarcoding. The abundances are determined for all transects using summer samples with peak biomass. This approach provides important insights into the possibility of obtaining information on relative species abundances and evaluating this, including data from metabarcoding.

  • Insects identified in this context will also be used to complete the GBOL reference database. Since selected families of the megadiverse insect orders Hymenoptera, Coleoptera, Lepidoptera and Diptera are manually identified by EVK specialists, we expect many species that will complement the global DNA barcode reference libraries in the work process.

  • The study will provide an enormous data volume on flight active insect species by Malaise traps of selected sites. The combination of metabarcoding and selective abundance evaluations will provide a hitherto unavailable basis for assessing the biodiversity of insect species in nature reserves in Germany.

  • Participation in the evaluation of the results and the synthesis

Contact: Entomologischer Verein Krefeld
         Marktstraße 159
         D 47798 Krefeld

         post@entomologica.de

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